Search and Browse
Download
Enter Data
| Solution Information | help | |
| Enzyme: | Dual specificity protein phosphatase 3 | |
| inhibitor: | BDBM46229 | |
| substrate: | n/a | |
| Solution Type: | Aqueous | |
| pH at Preparation: | n/a | |
| Temp. Prep.: | n/a | |
| Comments: | Assay materials: 1) VHR1 was obtained from the assay provider's laboratory. 2) Assay Buffer: 20mM Bis-Tris pH 6.0, 1mM DTT, 0.005% Tween-20 3) Biomol Green - Biomol (Cat #AK111-1000) VHR1 dose-response protocol 1) Dispense 10ul of assay buffer into columns 1 and 2 of Greiner 384-well clear plates (#781101). 2) Add 10ul of 250uM pNPP in water to all wells 3) Dispense serially diluted compounds to columns 3-22 and DMSO to columns 1-2 and 23-24. Final concentration DMSO in the assay is 1.575% 4) Dispense 10ul of 40nM VHR1 in assay buffer to wells in columns 3-24. 5) Incubate lidded plate for 1 hour at room temp. 6) Add 20uL of Biomol to all wells 7) Incubate for 30 min to allow Biomol signal to develop 8) Read plate on M5 plate reader in absorbance mode at 620 nm. | |
| If you find an error in this entry please send us an E-mail | ||
|
Home |
| |
Search |
| |
Deposit |
| |
SiteMap |
| |
About us |
| |
Email us |
| |
Info |
|
©2000 BindingDB. All rights reserved. |
|||||||||||||